Novel methods to study intestinal microbiota

Abstract

This thesis discusses the culture-dependent and culture-independent methods for the analysis of the human intestinal microbiota, with a special focus on the New Generation Sequecing procedures. The discussion includes the comparission of the principles, advantages, and disadvantages of these techniques. These techniques include conventional methods and novel methods which depend on molecular sequencing, such as the first generation and next generation sequencing techniques with different platforms of next generation sequencing tecnologies. Library data collection methods were used such as books, textbooks, scientific journal articles, and online library databases such as ISI Web of Science, Bibsys, PubMed, Google Scholar, and Google were used for this literature review paper. In conclusion, choosing among the increasing number of methods of analyzing human intestinal microbiota depends upon the goal and the target that is needed to be achived; sinces advantages and disadvantages are associated with all of these methods.