dc.contributor.advisor | Divon, Hege | |
dc.contributor.advisor | Skaar, Hanne Ida | |
dc.contributor.author | Henriksen, Erik Magnus Nedland | |
dc.coverage.spatial | Norway | en_US |
dc.date.accessioned | 2022-03-09T14:41:33Z | |
dc.date.available | 2022-03-09T14:41:33Z | |
dc.date.issued | 2021 | |
dc.identifier.uri | https://hdl.handle.net/11250/2984093 | |
dc.description.abstract | Azoles are used today against fungal infections both clinically in humans and in agriculture to great effect. However, recent years have seen the rise of azole-resistant fungi. The two most common mutations causing the increased resistance are the TR34/L98H and TR46/Y121F/T289A mutations located in the cyp51A gene. One of the species with increasing azole resistance is the common pathogen Aspergillus fumigatus. While other countries have conducted surveys to gauge the level of azole resistant A. fumigatus, Norway has not. The BARNES project sought to provide preliminary data on how widespread azole-resistant A. fumigatus are in farms across Norway, in addition to serving as a pilot project for a larger study. Citizen science was used to sample A. fumigatus from farms in Norway. These samples were identified as A. fumigatus by calmodulin sequencing and screened for resistance to itraconazole, voriconazole, and posaconazole with VIPCheckTM and then E-tests. The cyp51A gene was sequenced in resistant isolates. Of the 108 isolates of A. fumigatus gathered, four (3,7%) were resistant to all three azoles. Two of the resistant isolates had mutations in the cyp51A gene, one with the TR34/L98H mutation and the other with the TR46/Y121F/T289A mutation. This is the first time the TR46/Y121F/T289A mutation has been proven to exist environmentally in Norway. The other two isolates did not have any mutations in the cyp51A gene. While one is most likely actually a sensitive isolate, the other must owe its resistance to a mechanism other than a cyp51A mutation. While the percentage of resistant isolates are in line with data from other countries, a study with a larger data sample is needed to accurately reflect the occurrence of azole-resistant A. fumigatus in Norway. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Norwegian University of Life Sciences, Ås | en_US |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no | * |
dc.title | Pathogenic fungi in Norway : screening for azole-resistant Aspergillus fumigatus in Norwegian barns | en_US |
dc.title.alternative | Patogene sopp i Norge : undersøkelse etter azolresistent Aspergillus fumigatus i norske gårder | en_US |
dc.type | Master thesis | en_US |
dc.description.localcode | M-KB | en_US |