dc.contributor.author | Kinn, Steffan Kvilhaug | |
dc.date.accessioned | 2015-08-06T10:47:54Z | |
dc.date.available | 2015-08-06T10:47:54Z | |
dc.date.copyright | 2015 | |
dc.date.issued | 2015-08-06 | |
dc.identifier.uri | http://hdl.handle.net/11250/295186 | |
dc.description.abstract | A non-destructive protocol was created for extracting, isolating and detecting cyclotides from cultivated T.
Officinale flower heads. Optimal extraction was achieved by maceration for 15 minutes in 50% MeOH and
steeping plant material at 70 °C for 3 hours. Size exclusion chromatography was applied successfully using a
stationary phase with a molecular cut off at 1000-5000 Da yielding a good separation at 280nm. A molar
attenuation threshold was calculated from a protein standard with purified Kalata B1 and used to validate
isolated fractions. An amber colored fraction containing 0.39 mM protein was applied to a 400 MHz NMR to
determine the presence of cyclotides using extraordinary chemical shifts. Kalata B1 was not confirmed but
NMR showed fingerprint similarities to the standard and a signal at -0.1 ppm. This work demonstrates the
viability of the protocol for future use. | nb_NO |
dc.language.iso | eng | nb_NO |
dc.publisher | Norwegian University of Life Sciences, Ås | |
dc.rights | Navngivelse-Ikkekommersiell-IngenBearbeidelse 3.0 Norge | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/no/ | * |
dc.subject | Taraxacum Officinale | nb_NO |
dc.subject | Cyclotide | nb_NO |
dc.subject | NMR | nb_NO |
dc.title | Optimization of Cyclotide extraction from Taraxacum officinale flowers | nb_NO |
dc.type | Master thesis | nb_NO |
dc.subject.nsi | VDP::Technology: 500::Biotechnology: 590 | nb_NO |
dc.subject.nsi | VDP::Mathematics and natural science: 400::Chemistry: 440::Analytical chemistry: 445 | nb_NO |
dc.source.pagenumber | 81 | nb_NO |
dc.description.localcode | M-BIOTEK | nb_NO |