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dc.contributor.advisorRudi, Knut
dc.contributor.advisorNilsen, Morten
dc.contributor.authorWilborn, Lene Marie
dc.date.accessioned2021-12-13T09:40:40Z
dc.date.available2021-12-13T09:40:40Z
dc.date.issued2021
dc.identifier.urihttps://hdl.handle.net/11250/2833936
dc.description.abstractEarly colonization of beneficial bacteria in the infant gut is key for the development of healthy gut microbiota, immune system and for health later in life. Microbes are shared between mothers and their children. There is an ongoing debate about whether the first transmission occurs inside the uterus or after rupture of the amniotic membrane. Therefore, there still lacks information regarding the time of transmission and mechanisms for transfer. Previous studies have identified an association of Bacteroides in mother-child pairs. Therefore, this study aimed to identify vertical transmission of Bacteroides species from mother to child. Overlap of Bacteroides in 144 mother-child pairs in relation with mode of delivery were investigated. Ninety-five mother-child pairs were related to vaginal delivery, and 48 mother-child pairs were related to caesarean section delivery. Samples from mother’s stool, meconium, skin from newborns, and feces from 3 months old infants were analyzed to determine bacterial overlap. Material included in this study were received from the PreventADALL cohort. Bacterial composition in samples was studied by Illumina sequencing using the 16S rRNA gene and the rpoB gene. Amplicon sequence variant (ASV) profiles were used to study overlap of Bacteroides species in mothers and children. Indication of live bacterial cells in meconium was identified by cultivation. Bacteroides was present in all sample categories and represented 26.4% of the composition in meconium from vaginal delivered infants. Most samples with presence of Bacteroides were related to vaginal delivered infants. B. vulgatus was the predominant species in most samples. Several species of Bacteroides were present in mother-child pairs, and B. vulgatus and B. dorei had a significant association (p<0.05) between presence in mother-child pairs and vaginal delivery. B. vulgatus discovered in infants could be directly linked to mother’s stool, which indicates mother’s gut as an important reservoir for mother-child transmission. In conclusion, the results support that B. vulgatus is vertically transmitted from mother to child by vaginal delivery and persists in the infant gut up until 3 months of age. These findings indicate a stable colonization of B. vulgatus in the infant gut. Further research is essential to investigate if B. vulgatus is stable in the infant gut for a longer period, and the effect of mode of delivery on infant gut composition.en_US
dc.description.abstractSammenhengen mellom menneskets tarmmikrobiota og helse og sykdom har blitt avdekket de siste årene. Tidlig kolonisering av gunstige bakterier i nyfødtes tarm er essensielt for utviklingen av en sunn tarmflora, immunsystemet og for helse senere i livet. Mikrober overføres fra mor til barn. En debatt pågår om hvorvidt den første overføringen skjer i livmoren eller når fostervannsmembranen blir brutt. Tiden for første overføring og mekanismene bak overføringen mangler derfor fremdeles. Tidligere studier har identifisert en assosiasjon av Bacteroides i mor-barn par. Målet til denne studien er derfor å identifisere en vertikal overføring av arter av Bacteroides fra mor til barn.en_US
dc.language.isoengen_US
dc.publisherNorwegian University of Life Sciences, Åsen_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/deed.no*
dc.subjectBacteroidesen_US
dc.titleVertical transmission of Bacteroides in mother-child pairs in relation to vaginal deliveryen_US
dc.title.alternativeVertikal overføring av Bacteroides i mor-barn par i sammenheng med vaginal fødselen_US
dc.typeMaster thesisen_US
dc.description.localcodeM-BIOTEKen_US


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Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal
Med mindre annet er angitt, så er denne innførselen lisensiert som Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal