Biofilm production among pseudomonas from the food industry
Master thesis
Permanent lenke
http://hdl.handle.net/11250/216870Utgivelsesdato
2014-08-07Metadata
Vis full innførselSamlinger
- Master's theses (KBM) [891]
Sammendrag
Pseudomonas are gram-negative bacteria which show great diversity of metabolic activity
and are found in a variety of niches. Existences of the Pseudomonas spp. in the biofilm
forms are posing problems in the food industries due to their potential of contaminating food
and the food products. These unique microbial structures are more resistant to physical and
chemical stress than the planktonic form of life. Therefore, Pseudomonas spp. is of great
concern particularly to the food industries.
The study is intended to achieve the greater knowledge on the biofilm formation by
Pseudomonas spp. and the enzymatic action to prevent it. Twenty- seven different
Pseudomonas strains from different food processing plants and reference laboratory, were
tested. Identification of the strains were performed by API 20NE methodology. These strains
were subjected to form biofilm in laboratory conditions. The study focused on capacity of
the individual strain to form biofilm and the effectiveness of the disinfectant and enzymes to
inhibit the biofilm produced by the strains.
In the study, four Pseudomonas strains out of twenty-seven were identified differently from
that of 16 s RNA technology. Different Pseudomonas species showed different level of
biofilm in different cultural conditions but the best biofilm formation have been found in
TSBNG medium. Pseudomonas fluoresecens (3831) Pseudomonas putida (2.12) and
Pseudomonas fragi (96.6) stood the best among biofilm forming Pseudomonas strains.
In addition, Dispersin B, Proteinase K, DNase and Chlorine, were applied to inhibit biofilm
produced by the different Pseudomonas strains, where activities of these compounds were
found to be less effective.
The findings of the present study showed that enzymes are very low effective in inhibition
of biofilm produced by Pseudomonas strains.