Transcriptome changes in diploid and triploid Atlantic cod (Gadus- morhua) in response to phenylhydrazine-induced anaemia

Abstract

Anaemia is a common pathology associated with many diseases and infections. Hemolytic anaemia induced by Phenylhydrazine induce anaemia is a model which has been applied widely on animal including fish. The Atlantic cod is an important economic fish but research about anaemia, the physical reaction and genetic mechanism of fish reacted with the anaemia is not well known. Diploid and triploid was induced anaemia by injected 5mg. kg-1 Phenylhydrazin and collected blood, spleen and head kidney samples after 24, 72 and 168 hours post injection. The haematocrit observed after 24, 72 and 168 hour reduced significantly. There was no evident in genes regulation shows that the fish could recover after injected. Research on transcription changes in anaemia Atlantic cod was done by combine micro array analysis, qPCR analysis to analyse the genes transcription changes in side the fish thus predict the animal situation and physical changes. A large number of unregulated and down regulated of gene in different pathway was recorded. At day 3, of experiment, the initial of up regulated immune gene occurred in spleen and blood. At day 7, almost all immune gene was significant increased transcription, beside that genes involved in cell protection again inflammation, stress and nutrient metabolism gene are also up regulated. Data collected on day 7 samples was high correlated; at this time point , the fish seem tried to protect them self again internal inflammation. Genes code protein, which contribute to maintain cell structure and function down regulated. Blood smear analysis recoded a significant increase of various leucocytes appeared in blood for immunise activities. The transcription information can be use for further research in biological and physical pathway of fish under anaemia condition.