Vis enkel innførsel

dc.contributor.advisorHarvey, Thomas Nelson
dc.contributor.advisorBoyartchuk, Victor
dc.contributor.advisorTetens, Jens
dc.contributor.authorNalpadan, Avon Augustin
dc.date.accessioned2022-10-25T08:36:55Z
dc.date.available2022-10-25T08:36:55Z
dc.date.issued2022
dc.identifier.urihttps://hdl.handle.net/11250/3028118
dc.description.abstractCas9 expressing MDBK (Madin-Darby bovine kidney) cell lines were produced for a GeCKO (genome scale CRISPR/Cas9 gene knockout) screening against Bovine diseases. The lines were transduced with lentiviral vectors containing Cas9 and quantified for RNA expression and protein levels. sgRNAs were designed and assembled into lentiviral vectors. Transduction of Cas9 expressing MDBK cell lines with sgRNA containing lentivirus resulted in CRISPR/Cas9 gene editing. Editing efficiencies of selected lines were compared and correlated with their Cas9 expression profiles.en_US
dc.language.isoengen_US
dc.publisherNorwegian University of Life Sciences, Åsen_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/deed.no*
dc.subjectMDBKen_US
dc.subjectGeCKOen_US
dc.subjectCRISPR/Cas9en_US
dc.subjectGene editingen_US
dc.subjectBovine diseasesen_US
dc.titleDeveloping Cas9 expressing MDBK cell lines for GeCKO screening against bovine diseasesen_US
dc.typeMaster thesisen_US
dc.description.localcodeEM-ABGen_US


Tilhørende fil(er)

Thumbnail

Denne innførselen finnes i følgende samling(er)

Vis enkel innførsel

Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal
Med mindre annet er angitt, så er denne innførselen lisensiert som Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal