Developing Cas9 expressing MDBK cell lines for GeCKO screening against bovine diseases

dc.contributor.advisor	Harvey, Thomas Nelson
dc.contributor.advisor	Boyartchuk, Victor
dc.contributor.advisor	Tetens, Jens
dc.contributor.author	Nalpadan, Avon Augustin
dc.date.accessioned	2022-10-25T08:36:55Z
dc.date.available	2022-10-25T08:36:55Z
dc.date.issued	2022
dc.identifier.uri	https://hdl.handle.net/11250/3028118
dc.description.abstract	Cas9 expressing MDBK (Madin-Darby bovine kidney) cell lines were produced for a GeCKO (genome scale CRISPR/Cas9 gene knockout) screening against Bovine diseases. The lines were transduced with lentiviral vectors containing Cas9 and quantified for RNA expression and protein levels. sgRNAs were designed and assembled into lentiviral vectors. Transduction of Cas9 expressing MDBK cell lines with sgRNA containing lentivirus resulted in CRISPR/Cas9 gene editing. Editing efficiencies of selected lines were compared and correlated with their Cas9 expression profiles.	en_US
dc.language.iso	eng	en_US
dc.publisher	Norwegian University of Life Sciences, Ås	en_US
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no	*
dc.subject	MDBK	en_US
dc.subject	GeCKO	en_US
dc.subject	CRISPR/Cas9	en_US
dc.subject	Gene editing	en_US
dc.subject	Bovine diseases	en_US
dc.title	Developing Cas9 expressing MDBK cell lines for GeCKO screening against bovine diseases	en_US
dc.type	Master thesis	en_US
dc.description.localcode	EM-ABG	en_US

Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal