A Research-Inspired Biochemistry Laboratory Module - Combining Expression, Purification, Crystallization, Structure-Solving, and Characterization of a Flavodoxin-like Protein
Peer reviewed, Journal article
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Original versionBiochemistry and Molecular Biology Education. 2019, 47 (3), 318-332. 10.1002/bmb.21218
Many laboratory courses consist of short and seemingly unconnected individual laboratory exercises. To increase the course consistency, relevance, and student engagement, we havedevelopedaresearch-inspiredandproject-basedmodule, “From Gene to Structure and Function”. This 2.5-week full-day biochemistry and structural biology module covers protein expression, puriﬁcation, structure solving, and characterization.Themoduleiscenteredaroundthe ﬂavodoxin-likeprotein NrdI, involved in the activation of the bacterial ribonucleotide reductase enzyme system. Through an in-depth focus on one speciﬁc protein, the students will learn the basic laboratory skills needed in order to generate a broader knowledge and breadth within the ﬁeld. With respect to generic skills, the studentsreporttheir ﬁndingsasascientiﬁcarticle,withtheaim to learn to present concise research results and write scientiﬁc papers. The current research-inspired project has the potential ofbeingfurtherdevelopedintoamorediscovery-drivenproject and extended to include other molecular biological techniques orbiochemical/biophysicalcharacterizations.Instudentevaluations, this research-inspired laboratory course has received very high ratings and been highly appreciated, where the students have gained research experience for more independent future work in the laboratory. © 2019 The Authors. Biochemistry and Molecular Biology Education published by Wiley Periodicals, Inc. on behalf of International Union of Biochemistry andMolecularBiology,47(3):318–332,2019.