The performance of Quantum blue phytase at different pH levels using wheat based diet in an in vitro simulation of poultry crop

Abstract

An experiment was carried out to investigate the impact of different dietary pH levels as stimulate the efficacy of Quantum blue phytase in an in vitro simulation of poultry crop. The experiment consisted of two parts; an acid trial and an in vitro experiment. In the acid trial, four organic acids; propionic acid, formic acid, acidic acid and citric acid were tested separately on two kinds of diet mixtures that made by 4g of barleybased diet or wheat-based diet with 12ml distilled water. Water to feed ratio was 3:1. Each acid was titrated on a diet modal to reach either pH 5.5, 4.5 or 3.5, respectively. The required dosages were noted. Then, modals prepared with corrected pH (5.5, 4.5 and 3.5) were incubated in 40 ℃ water bath for 1 hour. The pH ranges were recorded during incubation at 5min, 10min, 30min and 1hour to observe acids stabilities. As result, the formic acid was applied in the in vitro experiment.