Blar i Faculty of Biosciences (BioVit) på forfatter "Harvey, Thomas Nelson"
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Cloning and characterization of Cas9 in IPEC-J2 cells for CRISPR screens against porcine E. coli pathogen
Arntsen, Jostein (Master thesis, 2021)Bakgrunn Svin er ofte brukt husdyr i landbruket, brukt primært for dets kjøtt. Et ofte forekommende problem i svineindustrien er diare forårsaket av bakterier, hvor enterotoksinproduserende Escherichia coli(ETEC) er en ... -
Developing Cas9 expressing MDBK cell lines for GeCKO screening against bovine diseases
Nalpadan, Avon Augustin (Master thesis, 2022)Cas9 expressing MDBK (Madin-Darby bovine kidney) cell lines were produced for a GeCKO (genome scale CRISPR/Cas9 gene knockout) screening against Bovine diseases. The lines were transduced with lentiviral vectors containing ... -
Development of lentiviral technology for use in Atlantic salmon
Andreassen, Sara Stenmark (Master thesis, 2022)Lentiviral vectors are used to deliver and stably integrate genetic material into the genome of cells. When combined with CRISPR based gene editing technology, targeted editing of one or many host genes in a pooled format ... -
Pseudotyping lentiviral vectors for transduction of Salmo salar cells using glycoprotein from infectious salmon anaemia virus
Låstad, Thea Marie (Master thesis, 2023)Lentivirus is commonly used as vector for CRISPR applications. The common vector system utilises glycoproteins from the vesicular stomatitis virus (VSV-G). This vector has broad a tropism, including the salmonid species ...