Cecum microbiota in mice affected by selenium level in diet
Master thesis
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Date
2014-08-13Metadata
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- Master's theses (KBM) [927]
Abstract
Selenium (Se) level in diet has been seen to affect gastrointestinal microbiota in mice,
and microbiota has been seen to affect the uptake and expression of Se and
selenoproteins. In this study, cecum from mice fed with low Se diet and normal Se diet
has been examined, and their microbiota community composition compared. DNA
from 16 cecum samples from each diet was extracted and 16S Metagenomic
Sequencing was done with the Illiumina MiSeq Sequencer. Output was processed with
Qiime pipeline for alpha and beta diversity analyses. Significant difference in
abundance was found in two genera, Akkermansia and Oscillospira.