Evaluation of novel PCR‑based method to assess gill injuries in fish caused by the cnidarian Ectopleura larynx

Abstract

Gill disease is a major threat to aquaculture of Atlantic salmon, with an unknown and likely underestimated contribution from cnidarians such as jellyfish and biofouling hydroids. To better understand the risk and thus enable mitigation, technology for the certain identification of cnidarian-related gill damage is needed. We used the hydroid Ectopleura larynx in a case study to determine whether the exposure of salmon to nematocyst-bearing hydrozoans can be deducted via non-destructive PCR-based methods. In a field experiment, we evaluated (i) whether swabbing the inside of the gill operculum in farmed Salmo salar and subsequent PCR analysis can provide quantifiable information about the presence of E. larynx material in the gill chamber and, if so, (ii) whether the screening results correlate with histological assessments of gill damage. The developed PCR methods were able to detect the presence of biofouling hydroids in ambient water. However, despite exposure to suspended hydroid particle concentrations that did result in gill damage in some salmon, quantitative PCR results did not correlate with histological gill assessments. For opercular swabs to serve as a diagnostic tool for detecting biofouling-mediated gill damage in live salmon, increased specificity of genetic markers and improved sampling methods are needed. Animal trial permit no. 24252 (granted 06.07.2020, Norwegian Food Safety Authority). Gill disease · Atlantic salmon · Salmo salar · Biofouling · Net cleaning