dc.contributor.author | Bwalya, Patricia | |
dc.contributor.author | Hang'Ombe, Bernard M. | |
dc.contributor.author | Gamil, Amr Ahmed Abdelrahim | |
dc.contributor.author | Munang´andu, Hetron Mweemba | |
dc.contributor.author | Evensen, Øystein | |
dc.contributor.author | Mutoloki, Stephen | |
dc.date.accessioned | 2021-11-18T13:01:25Z | |
dc.date.available | 2021-11-18T13:01:25Z | |
dc.date.created | 2021-02-15T13:51:27Z | |
dc.date.issued | 2020 | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | https://hdl.handle.net/11250/2830324 | |
dc.description.abstract | The autovaccine was produced in-house using a bacterial isolate from a diseased fish from the target farm. Three groups of 150 fish each were injected with either 1) an oil-adjuvanted, inactivated whole cell autovaccine, 2) adjuvant only or 3) PBS (negative control). Approximately 660 degree days post vaccination, the fish were challenged with 9x105 cfu bacteria/fish by intraperitoneal injection and monitored for a further 28 days. Protection against infections was measured by lack of/reduced bacterial loads both by bacterial re-isolation and immunohistochemistry as well as absence of clinical signs/pathology. Significantly less L. garvieae (p<0.03) was re-isolated from either the adjuvant only or control groups compared to the vaccinated group. Furthermore, a significantly high amount (p<0.001) of anti-L. garvieae specific antibodies were observed in the vaccinated group compared to the adjuvant only or control groups at time of challenge. This coincided with protection against infection measured by absence/reduced L. garvieae re-isolation from internal organs, reduced clinical signs and lack of pathology in this group. In the adjuvant only and control groups, bacteria were re-isolated from the kidney, liver, spleen, brain and eyes during the first 14 days. The findings suggest that oil-based vaccines can protect tilapia against L. garvieae infection through an antibody mediated response. | |
dc.language.iso | eng | |
dc.title | A whole-cell Lactococcus garvieae autovaccine protects Nile tilapia against infection | |
dc.type | Peer reviewed | |
dc.type | Journal article | |
dc.description.version | publishedVersion | |
dc.source.volume | 15 | |
dc.source.journal | PLOS ONE | |
dc.source.issue | 3 | |
dc.identifier.doi | 10.1371/journal.pone.0230739 | |
dc.identifier.cristin | 1889936 | |
dc.relation.project | NORHED: TAN-13/0027 | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |