dc.contributor.author | Rhein-Knudsen, Nanna | |
dc.contributor.author | Guan, Chengran | |
dc.contributor.author | Mathiesen, Geir | |
dc.contributor.author | Horn, Svein Jarle | |
dc.date.accessioned | 2021-10-28T07:55:01Z | |
dc.date.available | 2021-10-28T07:55:01Z | |
dc.date.created | 2021-10-26T09:43:58Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Algal Research. 2021, 60 . | |
dc.identifier.issn | 2211-9264 | |
dc.identifier.uri | https://hdl.handle.net/11250/2826166 | |
dc.description.abstract | Brown seaweeds are rich in carbohydrates and may be used as a source of fermentable sugars. Saccharification of the seaweed biomass can be carried out enzymatically by a combination of cellulases and alginate lyases. In this study, thermotolerant exo- and endo alginate lyases were cloned and expressed in Bacillus subtilis. The lyases were secreted to the culture supernatant and used directly together with a commercial cellulase preparation to saccharify Saccharina latissima biomass. The results showed that the strategy of using the culture supernatants directly as a source of alginate lyases worked very well, releasing glucose, mannitol, and uronic acids. The ratio between the exo- and endo-acting alginate lyases proved to be very important for saccharification yield, and under optimal reaction conditions the use of culture supernatants containing alginate lyases improved final glucose concentration by 73%, when compared to only applying cellulases. This direct use of culture supernatants as a source of alginate lyases shows that enzyme purification steps are not needed, saving seaweed processing costs and points to the possibility of a relatively simple on-site enzyme production for seaweed biorefining. | |
dc.language.iso | eng | |
dc.title | Expression and production of thermophilic alginate lyases in Bacillus and direct application of culture supernatant for seaweed saccharification | |
dc.type | Peer reviewed | |
dc.type | Journal article | |
dc.description.version | publishedVersion | |
dc.source.pagenumber | 0 | |
dc.source.volume | 60 | |
dc.source.journal | Algal Research | |
dc.identifier.doi | 10.1016/j.algal.2021.102512 | |
dc.identifier.cristin | 1948446 | |
dc.relation.project | Norges forskningsråd: 294946 | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |