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dc.contributor.authorRhein-Knudsen, Nanna
dc.contributor.authorGuan, Chengran
dc.contributor.authorMathiesen, Geir
dc.contributor.authorHorn, Svein Jarle
dc.date.accessioned2021-10-28T07:55:01Z
dc.date.available2021-10-28T07:55:01Z
dc.date.created2021-10-26T09:43:58Z
dc.date.issued2021
dc.identifier.citationAlgal Research. 2021, 60 .
dc.identifier.issn2211-9264
dc.identifier.urihttps://hdl.handle.net/11250/2826166
dc.description.abstractBrown seaweeds are rich in carbohydrates and may be used as a source of fermentable sugars. Saccharification of the seaweed biomass can be carried out enzymatically by a combination of cellulases and alginate lyases. In this study, thermotolerant exo- and endo alginate lyases were cloned and expressed in Bacillus subtilis. The lyases were secreted to the culture supernatant and used directly together with a commercial cellulase preparation to saccharify Saccharina latissima biomass. The results showed that the strategy of using the culture supernatants directly as a source of alginate lyases worked very well, releasing glucose, mannitol, and uronic acids. The ratio between the exo- and endo-acting alginate lyases proved to be very important for saccharification yield, and under optimal reaction conditions the use of culture supernatants containing alginate lyases improved final glucose concentration by 73%, when compared to only applying cellulases. This direct use of culture supernatants as a source of alginate lyases shows that enzyme purification steps are not needed, saving seaweed processing costs and points to the possibility of a relatively simple on-site enzyme production for seaweed biorefining.
dc.language.isoeng
dc.titleExpression and production of thermophilic alginate lyases in Bacillus and direct application of culture supernatant for seaweed saccharification
dc.typePeer reviewed
dc.typeJournal article
dc.description.versionpublishedVersion
dc.source.pagenumber0
dc.source.volume60
dc.source.journalAlgal Research
dc.identifier.doi10.1016/j.algal.2021.102512
dc.identifier.cristin1948446
dc.relation.projectNorges forskningsråd: 294946
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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