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dc.contributor.authorWallmeroth, Niklas
dc.contributor.authorJeschke, Daniel
dc.contributor.authorSlane, Daniel
dc.contributor.authorNägele, Janine
dc.contributor.authorVeerabagu, Manikandan
dc.contributor.authorMira-Rodado, Virtudes
dc.contributor.authorBerendzen, Kenneth Wayne
dc.description.abstractIn plants, several developmental processes are co-coordinated by cytokinins via phosphorylation dependent processes of the Two-Component System (TCS). An outstanding challenge is to track phosphorelay flow from cytokinin perception to its molecular outputs, of which gene activation plays a major role. To address this issue, a kinetic-based reporter system was expounded to track TCS phosphorelay activity in vivo that can distinguish between basal and cytokinin dependent effects of overexpressed TCS members. The TCS phosphorelay can be positively activated by cytokinin and inhibited by pharmaceuticals or naturally interfering components. In this case we took advantage of the phosphohistidinephosphatase Arabidopsis Response Regulator (ARR) 22 and investigated its phosphocompetition with other TCS members in regulating promoters of ARR5 and WUS in Arabidopsis thaliana cell culture protoplasts. In congruency with the proposed function of ARR22, overexpression of ARR22 blocked the activation of all B-type ARRs in this study in a TCS dependent manner. Furthermore, this effect could not be mimicked by A-type response regulator overexpression or compensated by AHP overexpression. Compared to other reporter assays, ours mimicked effects previously observed only in transgenic plants for all of the TCS proteins studied, suggesting that it is possible to expose phosphocompetition. Thus, our approach can be used to investigate gene signaling networks involving the TCS by leveraging ARR22 as a TCS inhibitor along with B-type ARR overexpression.en_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleARR22 overexpression can suppress plant Two-Component Regulatory Systemsen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.source.journalPLOS ONEen_US
dc.relation.projectAndre: German Research Foundation (CRC 1101)en_US
dc.relation.projectAndre: Eberhard Karls Universität Tübingen (DE)en_US
cristin.unitnameInstitutt for plantevitenskap

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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal