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dc.contributor.authorvan Eerde, André
dc.contributor.authorGottschamel, Johanna
dc.contributor.authorBock, Ralph
dc.contributor.authorHansen, Kristine Eraker Aasland
dc.contributor.authorMunangandu, Hetron Mweemba
dc.contributor.authorDaniell, Henry
dc.contributor.authorClarke, Jihong Liu
dc.description.abstractDengue fever is a mosquito (Aedes aegypti) ‐transmitted viral disease that is endemic in more than 125 countries around the world. There are four serotypes of the dengue virus (DENV 1‐4) and a safe and effective dengue vaccine must provide protection against all four serotypes. To date, the first vaccine, Dengvaxia (CYD‐TDV), is available after many decades’ efforts, but only has moderate efficacy. More effective and affordable vaccines are hence required. Plants offer promising vaccine production platforms and food crops offer additional advantages for the production of edible human and animal vaccines, thus eliminating the need for expensive fermentation, purification, cold storage and sterile delivery. Oral vaccines can elicit humoral and cellular immunity via both the mucosal and humoral immune systems. Here, we report the production of tetravalent EDIII antigen (EDIII‐1‐4) in stably transformed lettuce chloroplasts. Transplastomic EDIII‐1‐4‐expressing lettuce lines were obtained and homoplasmy was verified by Southern blot analysis. Expression of EDIII‐1‐4 antigens was demonstrated by immunoblotting, with the EDIII‐1‐4 antigen accumulating to 3.45% of the total protein content. Immunological assays in rabbits showed immunogenicity of EDIII‐1‐4. Our in vitro gastrointestinal digestion analysis revealed that EDIII‐1‐4 antigens are well protected when passing through the oral and gastric digestion phases but underwent degradation during the intestinal phase. Our results demonstrate that lettuce chloroplast engineering is a promising approach for future production of an affordable oral dengue vaccine.en_US
dc.description.abstractProduction of tetravalent dengue virus envelope protein domain III based antigens in lettuce chloroplasts and immunologic analysis for future oral vaccine developmenten_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleProduction of tetravalent dengue virus envelope protein domain III based antigens in lettuce chloroplasts and immunologic analysis for future oral vaccine developmenten_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.source.journalPlant Biotechnology Journalen_US
dc.relation.projectNorges forskningsråd: 192510en_US
cristin.unitnameInstitutt for produksjonsdyrmedisin
cristin.unitnameInstitutt for basalfag og akvamedisin

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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal