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dc.contributor.authorMalik, Muhammad Salman
dc.contributor.authorBjørgen, Håvard
dc.contributor.authorKannimuthu, Dhamotharan
dc.contributor.authorWessel, Øystein
dc.contributor.authorKoppang, Erling Olaf
dc.contributor.authorDi Cicco, Emiliano
dc.contributor.authorHansen, Elisabeth Furuseth
dc.contributor.authorDahle, Maria
dc.contributor.authorRimstad, Espen
dc.date.accessioned2020-11-26T15:03:24Z
dc.date.available2020-11-26T15:03:24Z
dc.date.created2019-12-08T23:31:33Z
dc.date.issued2019
dc.identifier.issn1999-4915
dc.identifier.urihttps://hdl.handle.net/11250/2689848
dc.description.abstractPiscine orthoreovirus (PRV-1) can cause heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (Salmo salar). The virus targets erythrocytes in the acute peak phase, followed by cardiomyocytes, before the infection subsides into persistence. The persistent phase is characterized by high level of viral RNA, but low level of viral protein. The origin and nature of persistent PRV-1 are not clear. Here, we analyzed for viral persistence and activity in various tissues and cell types in experimentally infected Atlantic salmon. Plasma contained PRV-1 genomic dsRNA throughout an 18-week long infection trial, indicating that viral particles are continuously produced and released. The highest level of PRV-1 RNA in the persistent phase was found in kidney. The level of PRV-1 ssRNA transcripts in kidney was significantly higher than that of blood cells in the persistent phase. In-situ hybridization assays confirmed that PRV-1 RNA was present in erythroid progenitor cells, erythrocytes, macrophages, melano-macrophages and in some additional un-characterized cells in kidney. These results show that PRV-1 establishes a productive, persistent infection in Atlantic salmon and that erythrocyte progenitor cells are PRV target cells.en_US
dc.description.abstractErythroid Progenitor Cells in Atlantic Salmon (Salmo salar) May Be Persistently and Productively Infected with Piscine Orthoreovirus (PRV)en_US
dc.language.isoengen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleErythroid Progenitor Cells in Atlantic Salmon (Salmo salar) May Be Persistently and Productively Infected with Piscine Orthoreovirus (PRV)en_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.description.versionpublishedVersionen_US
dc.source.volume11en_US
dc.source.journalVirusesen_US
dc.source.issue9en_US
dc.identifier.doi10.3390/v11090824
dc.identifier.cristin1757991
dc.source.articlenumber824en_US
cristin.unitcode192,16,2,0
cristin.unitcode192,16,1,0
cristin.unitnameInstitutt for mattrygghet og infeksjonsbiologi
cristin.unitnameInstitutt for basalfag og akvamedisin
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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