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dc.contributor.authorStokke, Runar
dc.contributor.authorReeves, Eoghan
dc.contributor.authorDahle, Håkon
dc.contributor.authorFedøy, Anita-Elin
dc.contributor.authorViflot, Thomas Øfstegaard
dc.contributor.authorOnstad, Solveig L.
dc.contributor.authorVulcano, Francesca
dc.contributor.authorPedersen, Rolf Birger
dc.contributor.authorEijsink, Vincent
dc.contributor.authorSteen, Ida Helene
dc.date.accessioned2020-11-11T13:24:41Z
dc.date.available2020-11-11T13:24:41Z
dc.date.created2020-02-25T16:22:49Z
dc.date.issued2020
dc.identifier.citationFrontiers in Microbiology, 2020, 11, 249en_US
dc.identifier.issn1664-302X
dc.identifier.urihttps://hdl.handle.net/11250/2687431
dc.description.abstractDeep-sea hydrothermal vents are amongst the most extreme environments on Earth and represent interesting targets for marine bioprospecting and biodiscovery. The microbial communities in hydrothermal vents are often dominated by chemolithoautotrophs utilizing simple chemical compounds, though the full extent of their heterotrophic abilities is still being explored. In the bioprocessing industry, where degradation of complex organic materials often is a major challenge, new microbial solutions are heavily needed. To meet these needs, we have developed novel in situ incubators and tested if deployment of recalcitrant materials from fish farming and wood-pulping industries introduced changes in the microbial community structure in hot marine hydrothermal sediments. The incubation chambers were deployed in sediments at the Bruse vent site located within the Jan Mayen vent field for 1 year, after which the microbial populations in the chambers were profiled by 16S rRNA Ion Torrent amplicon sequencing. A total of 921 operational taxonomic units (OTUs) were assigned into 74 different phyla where differences in community structure were observed depending on the incubated material, chamber depth below the sea floor and/or temperature. A high fraction of putative heterotrophic microbial lineages related to cultivated members within the Thermotogales were observed. However, considerable fractions of previously uncultivated and novel Thermotogales and Bacteroidetes were also identified. Moreover, several novel lineages (e.g., members within the DPANN superphylum, unidentified archaeal lineages, unclassified Thermoplasmatales and Candidatus division BRC-1 bacterium) of as-yet uncultivated thermophilic archaea and bacteria were identified. Overall, our data illustrate that amendment of hydrothermal vent communities by in situ incubation of biomass induces shifts in community structure toward increased fractions of heterotrophic microorganisms. The technologies utilized here could aid in subsequent metagenomics-based enzyme discovery for diverse industries.en_US
dc.language.isoengen_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/deed.no*
dc.titleTailoring hydrothermal vent biodiversity towards improved biodiscovery using a novel in-situ enrichment strategyen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.description.versionpublishedVersionen_US
dc.source.volume11en_US
dc.source.journalFrontiers in Microbiologyen_US
dc.identifier.doi10.3389/fmicb.2020.00249
dc.identifier.cristin1797447
dc.source.articlenumber249en_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode2


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Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal
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