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dc.contributor.authorTemesgen, Tamirat Tefera
dc.contributor.authorBarlaam, Alessandra
dc.contributor.authorTysnes, Kristoffer
dc.contributor.authorRobertson, Lucy
dc.date.accessioned2020-11-11T11:19:19Z
dc.date.available2020-11-11T11:19:19Z
dc.date.created2020-03-11T21:51:27Z
dc.date.issued2020
dc.identifier.citationFood Microbiology, 89, August 2020, 103447en_US
dc.identifier.issn0740-0020
dc.identifier.urihttps://hdl.handle.net/11250/2687356
dc.description.abstractThe potential public health impact of foodborne parasites (FBP) transmitted via contaminated fresh produces indicates the necessity for robust and reliable laboratory methods for their detection and identification on this infection vehicle. Standardization of methods for detection of common FBP in fresh produce is to be expected and ensuring that the DNA extraction approach is most appropriate for the FBP of interest and for the matrix being analyzed is also important. Therefore, the aim of the present study was to compare the efficacy of two commercially available DNA extraction procedures, the UNEX-based method and DNeasy PowerSoil kit in the detection of three protozoan parasites, C. cayetanensis, C. parvum, and T. gondii, on contaminated berries. Oocysts of each parasite were spiked into the pellets of raspberry and blueberry washes. The spiked pellets were then randomly assigned to DNA extraction using either the PowerSoil or UNEX method, with DNA extraction with both methods performed by two independent analysts. The detection rate when berry washes were spiked with 20 oocysts of C. cayetanensis, T. gondii, and C. parvum was 95%, 85%, and 40%, respectively, when using the PowerSoil kit; whereas the equivalent results using the UNEX method were 55%, 60%, and 5%, respectively. In addition, significantly lower Cq values were achieved for each parasite in the samples spiked with 500 oocysts when the PowerSoil kit was used. Possible reasons for these results are discussed, and include the composition of both the beads and the buffers in each method.en_US
dc.language.isoengen_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/deed.no*
dc.titleComparative evaluation of UNEX-based DNA extraction for molecular detection of Cyclospora cayetanensis, Toxoplasma gondii, and Cryptosporidium parvum as contaminants of berriesen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.description.versionpublishedVersionen_US
dc.source.volume89en_US
dc.source.journalFood Microbiologyen_US
dc.identifier.doi10.1016/j.fm.2020.103447
dc.identifier.cristin1801226
dc.relation.projectNorges forskningsråd: 267430en_US
dc.source.articlenumber103447en_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal
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