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dc.contributor.authorEijsink, Vincent
dc.contributor.authorPetrovic, Dejan
dc.contributor.authorForsberg, Zarah
dc.contributor.authorMekasha, Sophanit
dc.contributor.authorKjendseth, Åsmund Røhr
dc.contributor.authorVarnai, Aniko
dc.contributor.authorBissaro, Bastien
dc.contributor.authorVaaje-Kolstad, Gustav
dc.date.accessioned2020-11-09T10:28:35Z
dc.date.available2020-11-09T10:28:35Z
dc.date.created2019-06-05T11:46:56Z
dc.date.issued2019
dc.identifier.citationBiotechnology for Biofuels. 2019, 12.58 1-16.en_US
dc.identifier.issn1754-6834
dc.identifier.urihttps://hdl.handle.net/11250/2686909
dc.description.abstractLytic polysaccharide monooxygenases (LPMOs) are abundant in nature and best known for their role in the enzymatic conversion of recalcitrant polysaccharides such as chitin and cellulose. LPMO activity requires an oxygen co‑substrate, which was originally thought to be O2, but which may also be H2O2. Functional characterization of LPMOs is not straightforward because typical reaction mixtures will promote side reactions, including auto‑catalytic inactivation of the enzyme. For example, despite some recent progress, there is still limited insight into the kinetics of the LPMO reaction. Recent discoveries concerning the role of H2O2 in LPMO catalysis further complicate the picture. Here, we review commonly used methods for characterizing LPMOs, with focus on benefits and potential pitfalls, rather than on technical details. We conclude by pointing at a few key problems and potential misconceptions that should be taken into account when interpreting existing data and planning future experiments.en_US
dc.language.isoengen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleOn the functional characterization of lytic polysaccharide monooxygenases (LPMOs)en_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.description.versionpublishedVersionen_US
dc.source.pagenumber1-16en_US
dc.source.volume12.58en_US
dc.source.journalBiotechnology for Biofuelsen_US
dc.identifier.doi10.1186/s13068-019-1392-0
dc.identifier.cristin1702905
dc.relation.projectNorges forskningsråd: 243663en_US
dc.relation.projectNorges forskningsråd: 247001en_US
dc.relation.projectNorges forskningsråd: 262853en_US
dc.relation.projectNorges forskningsråd: 249865en_US
dc.relation.projectNorges forskningsråd: 268002en_US
dc.relation.projectNorges forskningsråd: 240967en_US
dc.relation.projectNorges forskningsråd: 257622en_US
cristin.unitcode192,12,0,0
cristin.unitnameKjemi, bioteknologi og matvitenskap
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal