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dc.contributor.authorSalvesen, Øyvind
dc.contributor.authorEspenes, Arild
dc.contributor.authorReiten, Malin Rokseth
dc.contributor.authorVuong, Tram Thu
dc.contributor.authorMalachin, Giulia
dc.contributor.authorTran, Linh Dinh Thoai
dc.contributor.authorAndréoletti, Olivier
dc.contributor.authorOlsaker, Ingrid
dc.contributor.authorBenestad, Sylvie Lafond
dc.contributor.authorTranulis, Michael A.
dc.contributor.authorErsdal, Cecilie
dc.date.accessioned2020-10-26T09:05:46Z
dc.date.available2020-10-26T09:05:46Z
dc.date.created2020-01-14T09:22:03Z
dc.date.issued2020
dc.identifier.issn0928-4249
dc.identifier.urihttps://hdl.handle.net/11250/2684901
dc.description.abstractPrion diseases are progressive and fatal, neurodegenerative disorders described in humans and animals. According to the “protein-only” hypothesis, the normal host-encoded prion protein (PrPC) is converted into a pathological and infectious form (PrPSc) in these diseases. Transgenic knockout models have shown that PrPC is a prerequisite for the development of prion disease. In Norwegian dairy goats, a mutation (Ter) in the prion protein gene (PRNP) effectively blocks PrPC synthesis. We inoculated 12 goats (4 PRNP+/+, 4 PRNP+/Ter, and 4 PRNPTer/Ter) intracerebrally with goat scrapie prions. The mean incubation time until clinical signs of prion disease was 601 days post-inoculation (dpi) in PRNP+/+ goats and 773 dpi in PRNP+/Ter goats. PrPSc and vacuolation were similarly distributed in the central nervous system (CNS) of both groups and observed in all brain regions and segments of the spinal cord. Generally, accumulation of PrPSc was limited in peripheral organs, but all PRNP+/+ goats and 1 of 4 PRNP+/Ter goats were positive in head lymph nodes. The four PRNPTer/Ter goats remained healthy, without clinical signs of prion disease, and were euthanized 1260 dpi. As expected, no accumulation of PrPSc was observed in the CNS or peripheral tissues of this group, as assessed by immunohistochemistry, enzyme immunoassay, and real-time quaking-induced conversion. Our study shows for the first time that animals devoid of PrPC due to a natural mutation do not propagate prions and are resistant to scrapie. Clinical onset of disease is delayed in heterozygous goats expressing about 50% of PrPC levels.en_US
dc.language.isoengen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleGoats naturally devoid of PrPC are resistant to scrapieen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.description.versionpublishedVersionen_US
dc.source.volume51en_US
dc.source.journalVeterinary research (VR)en_US
dc.source.issue1en_US
dc.identifier.cristin1771989
cristin.unitcode192,16,3,0
cristin.unitcode192,16,1,0
cristin.unitnameInstitutt for produksjonsdyrmedisin
cristin.unitnameInstitutt for basalfag og akvamedisin
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode2


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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal