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dc.contributor.authorLindeman, Leif Christopher
dc.contributor.authorThaulow, Jens
dc.contributor.authorSong, You
dc.contributor.authorKamstra, Jorke H
dc.contributor.authorXie, Li
dc.contributor.authorAsselman, Jana
dc.contributor.authorAlestrøm, Peter
dc.contributor.authorTollefsen, Knut-Erik
dc.date.accessioned2020-10-20T13:10:43Z
dc.date.available2020-10-20T13:10:43Z
dc.date.created2019-12-11T13:36:58Z
dc.date.issued2019
dc.identifier.citationEnvironmental Epigenetics. 2019, 5 (3), .en_US
dc.identifier.issn2058-5888
dc.identifier.urihttps://hdl.handle.net/11250/2683948
dc.description.abstractThe water flea Daphnia magna is a keystone species in freshwater ecosystems and has been widely used as a model organism in environmental ecotoxicology. This aquatic crustacean is sensitive to environmental stressors and displays considerable plasticity in adapting to changing environmental conditions. Part of this plasticity may be due to epigenetic regulation of gene expression, including changes to DNA methylation and histone modifications. Because of the generally hypomethylated genome of this species, we hypothesized that the histone code may have an essential role in the epigenetic control and that histone modifications might be an early marker for stress. This study aims to characterize the epigenetic, transcriptional and phenotypic responses and their causal linkages in directly exposed adult (F0) Daphnia and peritoneal exposed neonates (F1) after a chronic (7-day) exposure to a sublethal concentration (10 mg/l) of 5-azacytidine, a well-studied vertebrate DNA methylation inhibitor. Exposure of the F0 generation significantly reduced the cumulative fecundity, accompanied with differential expression of genes in the one-carbon-cycle metabolic pathway. In the epigenome of the F0 generation, a decrease in global DNA methylation, but no significant changes on H3K4me3 or H3K27me3, were observed. In the F1 offspring generation, changes in gene expression, a significant reduction in global DNA methylation and changes in histone modifications were identified. The results indicate that exposure during adulthood may result in more pronounced effects on early development in the offspring generation, though interpretation of the data should be carefully done since both the exposure regime and developmental period is different in the two generations examined. The obtained results improve our understanding of crustacean epigenetics and the tools developed may promote use of epigenetic markers in hazard assessment of environmental stressors.en_US
dc.language.isoengen_US
dc.rightsNavngivelse-Ikkekommersiell 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/deed.no*
dc.titleEpigenetic, transcriptional and phenotypic responses in two generations of Daphnia magna exposed to the DNA methylation inhibitor 5-Azacytidineen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.description.versionpublishedVersionen_US
dc.source.pagenumber12en_US
dc.source.volume5en_US
dc.source.journalEnvironmental Epigeneticsen_US
dc.source.issue3en_US
dc.identifier.doi10.1093/eep/dvz016
dc.identifier.cristin1759322
dc.relation.projectNorges forskningsråd: 223268en_US
dc.relation.projectNorges forskningsråd: 221455en_US
cristin.unitcode192,14,0,0
cristin.unitcode192,16,1,0
cristin.unitnameMiljøvitenskap og naturforvaltning
cristin.unitnameInstitutt for basalfag og akvamedisin
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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Navngivelse-Ikkekommersiell 4.0 Internasjonal
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