dc.contributor.author | Telke, Amar | |
dc.contributor.author | Ovchinnikov, Kirill | |
dc.contributor.author | Vuoristo, Kiira | |
dc.contributor.author | Mathiesen, Geir | |
dc.contributor.author | Thorstensen, Tage | |
dc.contributor.author | Diep, Dzung B. | |
dc.date.accessioned | 2020-01-03T11:14:50Z | |
dc.date.available | 2020-01-03T11:14:50Z | |
dc.date.created | 2019-04-08T14:28:53Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | Frontiers Microbiology, 05 March 2019 | nb_NO |
dc.identifier.issn | 1664-302X | |
dc.identifier.uri | http://hdl.handle.net/11250/2634763 | |
dc.description.abstract | The leaderless bacteriocin Garvicin KS (GarKS) is a potent antimicrobial, being active against a wide range of important pathogens. GarKS production by the native producer Lactococcus garvieae KS1546 is, however, relatively low (80 BU/ml) under standard laboratory growth conditions (batch culture in GM17 at 30°C). To improve the production, we systematically evaluated the impact of different media and media components on bacteriocin production. Based on the outcomes, a new medium formulation was made that increased GarKS production about 60-fold compared to that achieved in GM17. The new medium was composed of pasteurized milk and tryptone (PM-T). GarKS production was increased further 4-fold (i.e., to 20,000 BU/ml) by increasing the gene dose of the bacteriocin gene cluster (gak) in the native producer. Finally, a combination of the newly composed medium (PM-T), an increased gene dose and cultivation at a constant pH 6 and a 50–60% dissolved oxygen level in growth medium, gave rise to a GarKS production of 164,000 BU/ml. This high production, which is about 2000-fold higher compared to that initially achieved in GM17, corresponds to a GarKS production of 1.2 g/L. To our knowledge, this is one of the highest bacteriocin production reported hitherto. | nb_NO |
dc.language.iso | eng | nb_NO |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no | * |
dc.title | Over 2000-Fold Increased Production of the Leaderless Bacteriocin Garvicin KS by Increasing Gene Dose and Optimization of Culture Conditions | nb_NO |
dc.type | Journal article | nb_NO |
dc.type | Peer reviewed | nb_NO |
dc.description.version | publishedVersion | nb_NO |
dc.source.volume | 10 | nb_NO |
dc.source.journal | Frontiers in Microbiology | nb_NO |
dc.identifier.doi | 10.3389/fmicb.2019.00389 | |
dc.identifier.cristin | 1690882 | |
dc.relation.project | Norges forskningsråd: 254784 | nb_NO |
cristin.unitcode | 192,12,0,0 | |
cristin.unitcode | 192,0,0,0 | |
cristin.unitname | Kjemi, bioteknologi og matvitenskap | |
cristin.unitname | Norges miljø- og biovitenskapelige universitet | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 2 | |