The use of Saccharomyces cerevisae CNCM I-1077 in feed for ruminants : effect on digestibility, rumen enviroment and methane emmission
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- Master’s theses (BioVit) 
The main objective of this experiment was to evaluate the effect of adding live yeast (Saccharomyces cerevisiae CNCM I-1077) in rations to dairy cows in early lactation on apparent total tract digestibility, rumen physiological parameters, methane emission, milk yield- and milk composition. Six rumen cannulated dairy cows of the breed Norwegian Red (NRF) in ≥ 2. lactation (average 35 days in milk at start) were blocked by: 1) days in milk 2) milk yield 3) body weight and divided in two groups: yeast + (Y+) and control (Y-). The animals were held in tie stalls at the Animal Production Experimental Centre, NMBU. The experimental design was a crossover design with two periods of 28 days, with a 14 days washout period in between. All animals were fed a high fiber (NDF) grass silage (520 g NDF/kg dry matter) in combination with a commercial concentrate as a total mixed ration (TMR). The experimental animals were fed three times a day, either 21 or 22 kg dry matter daily as a fixed amount. Each day at 09:00, the Y+ group had 1 x 1010 CFU/day Saccharomyces cerevisiae CNCM I-1077 inserted into the rumen through the rumen cannula. The Y- group had their cannula opened, but with no adding of yeast. Methane emission were measured for five days in each period by the ERUCT method with SF6 as marker. Daily milk yield, milk composition, rumen fermentation products, rumen pH, body condition score and body weight were registered. Apparent total tract digestibility was calculated from a 72-hour total collection of faeces and urine. There was in general no effect of dietary treatment (Y+/Y-) on nutrient digestibility, milk yield, milk composition or methane emission (P>0.05). There was a numerically higher rumen pH in the Y+ group compared to the Y- (P=0.11). There was difference between the Y+ and the Y- group on rumen fermentation pattern, with a trend (P=0.06) towards a higher concentration of total acids (mmol/L) in the Y- group. There was a significant effect of dietary treatment on rumen propionate (P=0.05) and butyrate (P=0.05) concentrations (mmol/L) with the highest concentrations in the Y- group. The results showed a trend (P=0.10) towards higher acetate concentrations in the Y- group compared to the Y+ group. There was no effect of treatment on rumen propionate, acetate or butyrate when measured as molar percentage, and no effect on the acetate:propionate ratio. This study found no effect of adding Saccharomyces cerevisiae CNCM I-1077 on nutrient digestibility, milk yield, milk composition or methane emission in dairy cows fed a high fiber grass silage and concentrate diet. However, Saccharomyces cerevisiae CNCM I-1077 may have an effect on rumen fermentation pattern and rumen pH.Formålet med dette forsøket var å evaluere effekten av å tilsette levende gjær (Saccharomyces cerevisiae CNCM I-1077) i rajonen til melkekyr i tidlig laktasjon på apparent total fordøyelighet, fysiologiske parameter i vom, metanutslipp, melkeytelse og kjemisk komposisjon i melk.