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dc.contributor.authorAlseth, Ingrun
dc.contributor.authorRognes, Torbjørn
dc.contributor.authorLindbäck, Toril
dc.contributor.authorSolberg, Inger
dc.contributor.authorRobertsen, Kristin
dc.contributor.authorKristiansen, Knut Ivan
dc.contributor.authorMainieri, Davide
dc.contributor.authorLillehagen, Lucy
dc.contributor.authorKolstø, Anne-Brit
dc.contributor.authorBjørås, M.
dc.date.accessioned2018-03-06T10:17:31Z
dc.date.available2018-03-06T10:17:31Z
dc.date.created2006-06-30T00:00:00Z
dc.date.issued2006
dc.identifier.citationMolecular Microbiology. 2006, 59 (5), 1602-1609.nb_NO
dc.identifier.issn0950-382X
dc.identifier.urihttp://hdl.handle.net/11250/2488831
dc.description.abstractSoil bacteria are heavily exposed to environmental methylating agents such as methylchloride and may have special requirements for repair of alkylation damage on DNA. We have used functional complementation of an Escherichia coli tag alkA mutant to screen for 3-methyladenine DNA glycosylase genes in genomic libraries of the soil bacterium Bacillus cereus. Three genes were recovered: alkC, alkD and alkE. The amino acid sequence of AlkE is homologous to the E. coli AlkA sequence. AlkC and AlkD represent novel proteins without sequence similarity to any protein of known function. However, iterative and indirect sequence similarity searches revealed that AlkC and AlkD are distant homologues of each other within a new protein superfamily that is ubiquitous in the prokaryotic kingdom. Homologues of AlkC and AlkD were also identified in the amoebas Entamoeba histolytica and Dictyostelium discoideum, but no other eukaryotic counterparts of the superfamily were found. The alkC and alkD genes were expressed in E. coli and the proteins were purified to homogeneity. Both proteins were found to be specific for removal of N-alkylated bases, and showed no activity on oxidized or deaminated base lesions in DNA. B. cereus AlkC and AlkD thus define novel families of alkylbase DNA glycosylases within a new protein superfamily.
dc.language.isoengnb_NO
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/deed.no*
dc.titleA new protein superfamily includes two novel 3-methyladenine DNA glycosylases from Bacillus cereus, AlkC and AlkDnb_NO
dc.title.alternativeA new protein superfamily includes two novel 3-methyladenine DNA glycosylases from Bacillus cereus, AlkC and AlkDnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionpublishedVersion
dc.source.pagenumber1602-1609nb_NO
dc.source.volume59nb_NO
dc.source.journalMolecular Microbiologynb_NO
dc.source.issue5nb_NO
dc.identifier.cristin616852
cristin.unitcode192,16,2,0
cristin.unitnameInstitutt for mattrygghet og infeksjonsbiologi
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode2


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Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal
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