Biogas production from food waste via co-digestion and digestion-effects on performance and microbial ecology

Abstract

In this work, performance and microbial structure of a digestion (food waste-only) and a co-digestion process (mixture of cow manure and food waste) were studied at mesophilic (37°C) and thermophilic (55°C) temperatures. The highest methane yield (480mL/g VS) was observed in the mesophilic digester (MDi) fed with food waste alone. The mesophilic co-digestion of food waste and manure (McoDi) yielded 26% more methane than the sum of individual digestions of manure and food waste. The main volatile fatty acid (VFA) in the mesophilic systems was acetate, averaging 93 and 172mg/L for McoDi and MDi, respectively. Acetate (2150mg/L) and propionate (833mg/L) were the main VFAs in the thermophilic digester (TDi), while propionate (163mg/L) was the major VFA in the thermophilic co-digester (TcoDi). The dominant bacteria in MDi was Chlorofexi (54%), while Firmicutes was dominant in McoDi (60%). For the mesophilic reactors, the dominant archaea was Methanosaeta in MDi, while Methanobacterium and Methanosaeta had similar abundance in McoDi. In the thermophilic systems, the dominant bacteria were Thermotogae, Firmicutes and Synergistetes in both digesters, however, the relative abundance of these phyla were diferent. For archaea, the genus Methanothermobacter were entirely dominant in both TDi and TcoDi.