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Genetic distribution of 295 Bacillus cereus group members based on adk-screening in combination with MLST (Multilocus Sequence Typing) used for validating a primer targeting a chromosomal locus in B. anthracis

dc.contributor.authorOlsen, Jaran S.
dc.contributor.authorSkogan, Gunnar
dc.contributor.authorFykse, Else Marie
dc.contributor.authorRawlinson, Elizabeth L. A.
dc.contributor.authorTomaso, Herbert
dc.contributor.authorGranum, Per Einar
dc.contributor.authorBlatny, Janet
dc.date.accessioned2017-10-10T09:02:28Z
dc.date.available2017-10-10T09:02:28Z
dc.date.created2008-01-25T00:00:00Z
dc.date.issued2007
dc.identifier.citationJournal of Microbiological Methods. 2007, 71 (3), 265-274.nb_NO
dc.identifier.issn0167-7012
dc.identifier.urihttp://hdl.handle.net/11250/2459366
dc.description.abstractThe genetic distribution of 295 Bacillus cereus group members has been investigated by using a modified Multilocus Sequence Typing method (MLST). By comparing the nucleic acid sequence of the adk gene fragment, isolates of B. cereus group members most related to B. anthracis may be easily identified. The genetic distribution, with focus on the B. anthracis close neighbours, was used to evaluate a new primer set for specific identification of B. anthracis. This primer set, BA5510-1/2, targeted the putative B. anthracis specific gene BA5510. Real-time PCR using BA5510-1/2 amplified the target fragment from all B. anthracis strains tested and only two (of 289) non-B. anthracis strains analysed. This is one of the most thoroughly validated chromosomal B. anthracis markers for real-time PCR identification, in which the screened collection contained several very closely related B. anthracis strains.nb_NO
dc.description.abstractGenetic distribution of 295 Bacillus cereus group members based on adk-screening in combination with MLST (Multilocus Sequence Typing) used for validating a primer targeting a chromosomal locus in B. anthracisnb_NO
dc.language.isoengnb_NO
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/deed.no*
dc.titleGenetic distribution of 295 Bacillus cereus group members based on adk-screening in combination with MLST (Multilocus Sequence Typing) used for validating a primer targeting a chromosomal locus in B. anthracisnb_NO
dc.title.alternativeGenetic distribution of 295 Bacillus cereus group members based on adk-screening in combination with MLST (Multilocus Sequence Typing) used for validating a primer targeting a chromosomal locus in B. anthracisnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionacceptedVersionnb_NO
dc.source.pagenumber265-274nb_NO
dc.source.volume71nb_NO
dc.source.journalJournal of Microbiological Methodsnb_NO
dc.source.issue3nb_NO
dc.identifier.doi10.1016/j.mimet.2007.10.001
dc.identifier.cristin617452
cristin.unitcode192,16,2,0
cristin.unitnameInstitutt for mattrygghet og infeksjonsbiologi
cristin.ispublishedtrue
cristin.fulltextpostprint
cristin.qualitycode1


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Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal
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