Browsing Brage NMBU by Author "Hamre, Anne Grethe"
Now showing items 1-10 of 10
-
Enzyme processivity changes with the extent of recalcitrant polysaccharide degradation
Hamre, Anne Grethe; Lorentzen, Silje Benedicte; Väljamäe, Priit; Sørlie, Morten (Peer reviewed; Journal article, 2014): Polysaccharide depolymerization in nature is primarily accomplished by processive glycoside hydrolases which abstract single carbohydrate chains from polymer crystals and cleave glycosidic bonds without dissociating from ... -
Kinetic relationships with processivity in Serratia marcescens family 18 glycoside hydrolases
Hamre, Anne Grethe; Sørlie, Morten (Peer reviewed; Journal article, 2019)In nature, recalcitrant polysaccharides such as chitin and cellulose are degraded by glycoside hydrolases (GH) that act synergistically through different modes of action including attack from reducing-end and nonreducing-end ... -
Ligninvalorisering ved bruk av enzymatiske cocktailer
Trondsen, Susann (Master thesis, 2018)Planters biomasse består i hovedsak av cellulose, hemicellulose og lignin, der lignin utgjør 15-40 % av plantens tørrvekt. Lignin er den nest mest forekommende polymeren på landjorda. Den er svært heterogen, avledet fra ... -
Processivity, substrate positioning, and binding: The role of polar residues in a family 18 glycoside hydrolase
Hamre, Anne Grethe; Suvamay, Jana; Reppert, Nicole; Payne, Christina M.; Sørlie, Morten (Journal article; Peer reviewed, 2015) -
Roles of charged residues in the active site of Lytic Polysaccharide Monooxygenase : effect on catalysis
Karlsen, Victoria (Master thesis, 2017)Lytic Polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes that catalyze the oxidative cleavage of glycosidic bonds in polysaccharides in the presence of H2O2 or molecular oxygen paired with a reductant. LPMOs ... -
The directionality of processive enzymes acting on recalcitrant polysaccharides is reflected in the kinetic signatures of oligomer degradation;
Hamre, Anne Grethe; Schaupp, Daniel; Eijsink, Vincent; Sørlie, Morten (Journal article; Peer reviewed, 2015) -
Thermodynamic aspects of processive enzymatic degradation of recalcitrant polysaccharides
Hamre, Anne Grethe (PhD Thesis;2015:46, Doctoral thesis, 2020-11-13)Carbohydrates play diverse, essential roles in all known organisms. These include roles in storage and structure as well as specific signaling roles. One carbohydrate can be linked to other carbohydrates or functional ... -
Thermodynamic Signatures of Substrate Binding for Three Thermobifida fusca Cellulases with Different Modes of Action
Hamre, Anne Grethe; Kaupang, Anita; Payne, Christina M.; Väljamäe, Priit; Sørlie, Morten (Peer reviewed; Journal article, 2019)The enzymatic breakdown of recalcitrant polysaccharides is achieved by synergistic enzyme cocktails of glycoside hydrolases (GHs) and accessory enzymes. Many GHs are processive, meaning that they stay bound to the substrate ... -
Treatment of recalcitrant crystalline polysaccharides with lytic polysaccharide monooxygenase relieves the need for glycoside hydrolase processivity
Hamre, Anne Grethe; Strømnes, Anne-Grethe; Gustavsen, Daniel; Vaaje-Kolstad, Gustav; Eijsink, Vincent; Sørlie, Morten (Peer reviewed; Journal article, 2019)Processive glycoside hydrolases associate with recalcitrant polysaccharides such as cellulose and chitin and repeatedly cleave glycosidic linkages without fully dissociating from the crystalline surface. The processive ... -
Uttrykk og karakterisering av en superoksid dismutase og en dihyrolipoamid dehydrogenase fra Thermobifida fusca
Al-Sadawi, Rim (Master thesis, 2018)Denne oppgaven er skrevet som en del av et større forskningsprosjekt med et overordnende mål om å tilegne seg kunnskap om ligninnedbrytende enzymer til jordbakterien Thermobifidia fusca. Lignocellulose består hovedsakelig ...