Browsing Brage NMBU by Author "Vaaje-Kolstad, Gustav"
Now showing items 1-20 of 53
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1H, 13C, 15N resonance assignment of the chitin-active lytic polysaccharide monooxygenase BlLPMO10A from Bacillus licheniformis
Courtade, Gaston; Balzer, Simone; Forsberg, Zarah; Vaaje-Kolstad, Gustav; Eijsink, Vincent; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2015)The chitin-active 19.2 kDa lytic polysaccharide monooxygenase BlLPMO10A from Bacillus licheniformis has been isotopically labeled and recombinantly expressed. In this paper, we report the 1H, 13C, 15N resonance assignment ... -
A small lytic polysaccharide monooxygenase from Streptomyces griseus targeting α- and β-chitin
Nakagawa, Yuko S.; Kudo, Madoka; Loose, Jennifer; Ishikawa, Takahiro; Totani, Kazuhide; Eijsink, Vincent; Vaaje-Kolstad, Gustav (Journal article; Peer reviewed, 2015) -
A trimodular bacterial enzyme combining hydrolytic activity with oxidative glycosidic bond cleavage efficiently degrades chitin
Mekasha, Sophanit; Tuveng, Tina Rise; Askarian, Fatemeh; Choudhary, Swati; Schmith-Dannert, Claudia; Niebisch, Axel; Modregger, Jan; Vaaje-Kolstad, Gustav; Eijsink, Vincent (Peer reviewed; Journal article, 2020) -
Analyzing Activities of Lytic Polysaccharide Monooxygenases by Liquid Chromatography and Mass Spectrometry
Westereng, Bjørge; Arntzen, Magnus Øverlie; Wittrup_Agger, Jane; Vaaje-Kolstad, Gustav (Journal article; Peer reviewed, 2017-04-18) -
Bacterial membrane-derived vesicles attenuate vancomycin activity against methicillin-resistant staphylococcus aureus
Kumaraswamy, Monika; Wiull, Kamilla; Joshi, Bishnu; Sakoulas, George; Kousha, Armin; Vaaje-Kolstad, Gustav; Johannessen, Mona; Hegstad, Kristin; Nizet, Victor; Askarian, Fatemeh (Peer reviewed; Journal article, 2021) -
Biochemical and structural characterisation of a family GH5 cellulase from endosymbiont of shipworm P. megotara
Junghare, Madan; Manavalan, Tamilvendan; Fredriksen, Lasse; Leiros, Ingar; Altermark, Bjørn; Eijsink, Vincent; Vaaje-Kolstad, Gustav (Peer reviewed; Journal article, 2023) -
Biochemical investigation of catalysis by lytic polysaccharide monooxygenases
Loose, Jennifer Sarah Maria (PhD Thesis;2016:69, Doctoral thesis, 2018-05-04)Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes that catalyze the oxidative cleavage of glycosidic bonds in the presence of dioxygen and an external electron donor. Currently, these enzymes are ... -
Calcium binding site in AA10 LPMO from Vibrio cholerae suggests modulating effects during environment survival and infection
Montserrat Canals, Mateu; Bjerregaard-Andersen, Kaare; Vinther Sørensen, Henrik; Cordara, Gabriele; Vaaje-Kolstad, Gustav; Krengel, Ute (Journal article, 2023) -
Characterization of a Glycoside hydrolase family 50 member PaGH50A from Pseudomonas aeruginosa and exploring the enzyme’s potential in modulating biofilm formation
Farah, Hibaq Ahmed (Master thesis, 2022)Pseudomonas aeruginosa is an opportunistic human pathogen that causes acute and chronic lung infections in individuals with compromised immune systems. The bacterium is wide spread in the environment and especially persistent ... -
Characterization of genes and proteins related to chitin metabolism in Atlantic salmon
Holen, Matilde Mengkrog (PhD Thesis;2022:46, Doctoral thesis, 2022)Chitin is an insoluble polysaccharide and important component in the extracellular matrix of invertebrates such as arthropods, algae, and fungi. In vertebrates, the role and significance of chitin have not been deeply ... -
Chemical shift assignments for the apo-form of the catalytic domain, the linker region, and the carbohydrate-binding domain of the cellulose-active lytic polysaccharide monooxygenase ScLPMO10C
Courtade, Gaston; Forsberg, Zarah; Vaaje-Kolstad, Gustav; Eijsink, Vincentius Gerardus Henricus; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2017) -
Chitinolytic enzymes contribute to the pathogenicity of Aliivibrio salmonicida LFI1238 in the invasive phase of cold-water vibriosis
Skåne, Anna; Edvardsen, Per Kristian Thorén; Cordara, Gabriele; Loose, Jennifer Sarah Maria; Leitl, Kira Daryl; Krengel, Ute; Sørum, Henning; Askarian, Fatemeh; Vaaje-Kolstad, Gustav (Peer reviewed; Journal article, 2022) -
Conversion of alpha-Chitin Substrates with Varying Particle Size and Crystallinity Reveals Substrate Preferences of the Chitinases and Lytic Polysaccharide Monooxygenase of Serratia marcescens
Nakagawa, Yuko S.; Eijsink, Vincent; Totani, K; Vaaje-Kolstad, Gustav (Journal article; Peer reviewed, 2013) -
Deteksjon og kvantifikasjon av HRV87 i A549 celler med og uten Neu5Ac in vitro
Ørstenvik, Hanne Lillerovde (Master thesis, 2021)Forkjølelse er et dagligdags problem som forårsakes av ulike varianter av rhinovirus. Rhinovirus er en av de vanligste årsakene til luftveisinfeksjoner og står for omlag 50 % av tilfellene. Forskningslitteraturen viser til ... -
Development of enzyme cocktails for complete saccharification of chitin using mono-component enzymes from Serratia marcescens
Mekasha, Sophanit; Byman, Ida Roksvåg; Lynch, Catherine; Toupalová, Hana; Andera, Ladislav; Næs, Tormod; Vaaje-Kolstad, Gustav; Eijsink, Vincentius Gerardus Henricus (Journal article; Peer reviewed, 2017)One potential strategy for biorefining of chitin-rich biomass entails enzymatic saccharification, which, so far, has been scarcely explored. Here, saccharification of chitin was explored using response surface methodology ... -
Discovery and characterization of a thermostable two-domain GH6 endoglucanase from a compost metagenome
Jensen, Marianne Slang; Fredriksen, Lasse; Mackenzie, Alasdair; Pope, Phillip; Leiros, Ingar; Chylenski, Piotr; Williamson, Adele Kim; Christopeit, Tony; Østby, Heidi; Vaaje-Kolstad, Gustav; Eijsink, Vincent (Journal article; Peer reviewed, 2018) -
Discovery and characterization of bacterial enzymes for processing of lignocellulosic biomass
Jensen, Marianne Slang (PhD Thesis;2019:62, Doctoral thesis, 2019)Utilization of non-edible biomass as a source of renewable carbon can assist the transition to a sustainable bioeconomy. Such biomass includes non-edible plant materials commonly referred to as lignocellulose. Lignocellulose ... -
Discovery and characterization of cellulose-active lytic polysaccharide monooxygenases
Forsberg, Zarah Kristina (PhD Thesis;2014:62, Doctoral thesis, 2014)The efficient depolymerization of lignocellulosic biomass to fermentable sugars by enzymatic hydrolysis is a key step in the transition towards a more environmentally friendly and sustainable bio-economy. However, the ... -
Discovery and characterization of enzymes acting on chitin
Tuveng, Tina Rise (PhD Thesis;2017:71, Doctoral thesis, 2017)In the shift from a fossil-based to a bio-based economy, exploration of renewable recourses is needed. Chitin is considered the second most abundant polysaccharide on Earth, after cellulose, and its water-soluble derivatives ... -
Discovery, expression, and characterization of novel laccases derived from marine bacteria
Neeraas, Priscilla Cassandra (Master thesis, 2019)Lignocellulose is the most abundant biomass on Earth and is primarily composed of three organic polymers; cellulose, hemicellulose, and lignin. Established technology exists to produce useful products such as fuels and ...