Enzymatic processing of lignocellulosic biomass: principles, recent advances and perspectives
Peer reviewed, Journal article
Published version
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https://hdl.handle.net/11250/2760359Utgivelsesdato
2020Metadata
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Originalversjon
Journal of Industrial Microbiology & Biotechnology. 2020, 47 623-657. 10.1007/s10295-020-02301-8Sammendrag
Efcient saccharifcation of lignocellulosic biomass requires concerted development of a pretreatment method, an enzyme cocktail and an enzymatic process, all of which are adapted to the feedstock. Recent years have shown great progress in most aspects of the overall process. In particular, increased insights into the contributions of a wide variety of cellulolytic and hemicellulolytic enzymes have improved the enzymatic processing step and brought down costs. Here, we review major pretreatment technologies and diferent enzyme process setups and present an in-depth discussion of the various enzyme types that are currently in use. We pay ample attention to the role of the recently discovered lytic polysaccharide monooxygenases (LPMOs), which have led to renewed interest in the role of redox enzyme systems in lignocellulose processing. Better understanding of the interplay between the various enzyme types, as they may occur in a commercial enzyme cocktail, is likely key to further process improvements.